Isolation of Avian influenza virus from polymerase chain reaction-negative cloacal samples of waterfowl.
Identifieur interne : 000C39 ( Main/Exploration ); précédent : 000C38; suivant : 000C40Isolation of Avian influenza virus from polymerase chain reaction-negative cloacal samples of waterfowl.
Auteurs : Mohamed E. El Zowalaty [États-Unis] ; Martha Abin ; Subhatra Raju ; Yogesh Chander ; Patrick T. Redig ; Hemmat K. Abd El Latif ; Mona A. El Sayed ; Sagar M. GoyalSource :
- Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc [ 1943-4936 ] ; 2011.
Descripteurs français
- KwdFr :
- ARN viral (), ARN viral (génétique), Animaux, Animaux sauvages, Cloaque (virologie), Embryon de poulet, Faux négatifs, Grippe chez les oiseaux (diagnostic), Grippe chez les oiseaux (virologie), Oiseaux, RT-PCR (médecine vétérinaire), Tests d'hémagglutination (), Tests d'hémagglutination (médecine vétérinaire), Virus de la grippe A (génétique), Virus de la grippe A (isolement et purification), Zoonoses (virologie).
- MESH :
- diagnostic : Grippe chez les oiseaux.
- génétique : ARN viral, Virus de la grippe A.
- isolement et purification : Virus de la grippe A.
- médecine vétérinaire : RT-PCR, Tests d'hémagglutination.
- virologie : Cloaque, Grippe chez les oiseaux, Zoonoses.
- ARN viral, Animaux, Animaux sauvages, Embryon de poulet, Faux négatifs, Oiseaux, Tests d'hémagglutination.
English descriptors
- KwdEn :
- Animals, Animals, Wild, Birds, Chick Embryo, Cloaca (virology), False Negative Reactions, Hemagglutination Tests (methods), Hemagglutination Tests (veterinary), Influenza A virus (genetics), Influenza A virus (isolation & purification), Influenza in Birds (diagnosis), Influenza in Birds (virology), RNA, Viral (chemistry), RNA, Viral (genetics), Reverse Transcriptase Polymerase Chain Reaction (veterinary), Zoonoses (virology).
- MESH :
- chemical , chemistry : RNA, Viral.
- diagnosis : Influenza in Birds.
- genetics : Influenza A virus, RNA, Viral.
- isolation & purification : Influenza A virus.
- methods : Hemagglutination Tests.
- veterinary : Hemagglutination Tests, Reverse Transcriptase Polymerase Chain Reaction.
- virology : Cloaca, Influenza in Birds, Zoonoses.
- Animals, Animals, Wild, Birds, Chick Embryo, False Negative Reactions.
Abstract
Avian influenza virus (AIV) is one of the most important zoonotic pathogens because of its potential to cause severe disease outbreaks in avian and human hosts. Virus isolation in embryonated chicken eggs (ECEs) remains a gold standard technique for AIV detection. However, some laboratories prefer molecular methods, such as real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR), for initial sample screening because of their high throughput sample processing and rapid results. Samples found positive on real-time qRT-PCR are then inoculated in ECEs for virus isolation and characterization. This approach is based on the premise that real-time qRT-PCR will detect all AIV-positive samples. The current study aimed to determine if AIV can be isolated from cloacal samples of waterfowl that were initially found to be negative by real-time qRT-PCR screening. Quantitative RT-PCR-negative cloacal samples (1,369) were inoculated for virus isolation in commercial nonspecific pathogen-free ECEs. After 4 days of incubation, the allantoic fluids were harvested and inoculated in fresh ECEs for a second passage. Allantoic fluids from 147 samples were positive for hemagglutination with chicken erythrocytes. Of the 147 hemagglutination-positive allantoic fluids, 82 were AIV positive when confirmed with real-time qRT-PCR. Ten isolates were subtyped as H7N2 (n = 7), H7N1, H1N2, and H2N2. In addition, N subtype could be determined for isolates from an additional 25 samples. These results highlight the fact that screening by real-time qRT-PCR may result in some false-negative cloacal samples for AIV.
DOI: 10.1177/104063871102300113
PubMed: 21217033
Affiliations:
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Le document en format XML
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El Zowalaty</name><affiliation wicri:level="1"><nlm:affiliation>Department of Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota, Saint Paul, MN 55108, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota, Saint Paul, MN 55108</wicri:regionArea><wicri:noRegion>MN 55108</wicri:noRegion></affiliation></author><author><name sortKey="Abin, Martha" sort="Abin, Martha" uniqKey="Abin M" first="Martha" last="Abin">Martha Abin</name></author><author><name sortKey="Raju, Subhatra" sort="Raju, Subhatra" uniqKey="Raju S" first="Subhatra" last="Raju">Subhatra Raju</name></author><author><name sortKey="Chander, Yogesh" sort="Chander, Yogesh" uniqKey="Chander Y" first="Yogesh" last="Chander">Yogesh Chander</name></author><author><name sortKey="Redig, Patrick T" sort="Redig, Patrick T" uniqKey="Redig P" first="Patrick T" last="Redig">Patrick T. Redig</name></author><author><name sortKey="Abd El Latif, Hemmat K" sort="Abd El Latif, Hemmat K" uniqKey="Abd El Latif H" first="Hemmat K" last="Abd El Latif">Hemmat K. Abd El Latif</name></author><author><name sortKey="El Sayed, Mona A" sort="El Sayed, Mona A" uniqKey="El Sayed M" first="Mona A" last="El Sayed">Mona A. El Sayed</name></author><author><name sortKey="Goyal, Sagar M" sort="Goyal, Sagar M" uniqKey="Goyal S" first="Sagar M" last="Goyal">Sagar M. 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El Zowalaty</name><affiliation wicri:level="1"><nlm:affiliation>Department of Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota, Saint Paul, MN 55108, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota, Saint Paul, MN 55108</wicri:regionArea><wicri:noRegion>MN 55108</wicri:noRegion></affiliation></author><author><name sortKey="Abin, Martha" sort="Abin, Martha" uniqKey="Abin M" first="Martha" last="Abin">Martha Abin</name></author><author><name sortKey="Raju, Subhatra" sort="Raju, Subhatra" uniqKey="Raju S" first="Subhatra" last="Raju">Subhatra Raju</name></author><author><name sortKey="Chander, Yogesh" sort="Chander, Yogesh" uniqKey="Chander Y" first="Yogesh" last="Chander">Yogesh Chander</name></author><author><name sortKey="Redig, Patrick T" sort="Redig, Patrick T" uniqKey="Redig P" first="Patrick T" last="Redig">Patrick T. Redig</name></author><author><name sortKey="Abd El Latif, Hemmat K" sort="Abd El Latif, Hemmat K" uniqKey="Abd El Latif H" first="Hemmat K" last="Abd El Latif">Hemmat K. Abd El Latif</name></author><author><name sortKey="El Sayed, Mona A" sort="El Sayed, Mona A" uniqKey="El Sayed M" first="Mona A" last="El Sayed">Mona A. El Sayed</name></author><author><name sortKey="Goyal, Sagar M" sort="Goyal, Sagar M" uniqKey="Goyal S" first="Sagar M" last="Goyal">Sagar M. Goyal</name></author></analytic><series><title level="j">Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc</title><idno type="eISSN">1943-4936</idno><imprint><date when="2011" type="published">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Animals, Wild</term><term>Birds</term><term>Chick Embryo</term><term>Cloaca (virology)</term><term>False Negative Reactions</term><term>Hemagglutination Tests (methods)</term><term>Hemagglutination Tests (veterinary)</term><term>Influenza A virus (genetics)</term><term>Influenza A virus (isolation & purification)</term><term>Influenza in Birds (diagnosis)</term><term>Influenza in Birds (virology)</term><term>RNA, Viral (chemistry)</term><term>RNA, Viral (genetics)</term><term>Reverse Transcriptase Polymerase Chain Reaction (veterinary)</term><term>Zoonoses (virology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN viral ()</term><term>ARN viral (génétique)</term><term>Animaux</term><term>Animaux sauvages</term><term>Cloaque (virologie)</term><term>Embryon de poulet</term><term>Faux négatifs</term><term>Grippe chez les oiseaux (diagnostic)</term><term>Grippe chez les oiseaux (virologie)</term><term>Oiseaux</term><term>RT-PCR (médecine vétérinaire)</term><term>Tests d'hémagglutination ()</term><term>Tests d'hémagglutination (médecine vétérinaire)</term><term>Virus de la grippe A (génétique)</term><term>Virus de la grippe A (isolement et purification)</term><term>Zoonoses (virologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>RNA, Viral</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Influenza in Birds</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Grippe chez les oiseaux</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Influenza A virus</term><term>RNA, Viral</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN viral</term><term>Virus de la grippe A</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Influenza A virus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Virus de la grippe A</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Hemagglutination Tests</term></keywords><keywords scheme="MESH" qualifier="médecine vétérinaire" xml:lang="fr"><term>RT-PCR</term><term>Tests d'hémagglutination</term></keywords><keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>Hemagglutination Tests</term><term>Reverse Transcriptase Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Cloaque</term><term>Grippe chez les oiseaux</term><term>Zoonoses</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Cloaca</term><term>Influenza in Birds</term><term>Zoonoses</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Animals, Wild</term><term>Birds</term><term>Chick Embryo</term><term>False Negative Reactions</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>ARN viral</term><term>Animaux</term><term>Animaux sauvages</term><term>Embryon de poulet</term><term>Faux négatifs</term><term>Oiseaux</term><term>Tests d'hémagglutination</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Avian influenza virus (AIV) is one of the most important zoonotic pathogens because of its potential to cause severe disease outbreaks in avian and human hosts. Virus isolation in embryonated chicken eggs (ECEs) remains a gold standard technique for AIV detection. However, some laboratories prefer molecular methods, such as real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR), for initial sample screening because of their high throughput sample processing and rapid results. Samples found positive on real-time qRT-PCR are then inoculated in ECEs for virus isolation and characterization. This approach is based on the premise that real-time qRT-PCR will detect all AIV-positive samples. The current study aimed to determine if AIV can be isolated from cloacal samples of waterfowl that were initially found to be negative by real-time qRT-PCR screening. Quantitative RT-PCR-negative cloacal samples (1,369) were inoculated for virus isolation in commercial nonspecific pathogen-free ECEs. After 4 days of incubation, the allantoic fluids were harvested and inoculated in fresh ECEs for a second passage. Allantoic fluids from 147 samples were positive for hemagglutination with chicken erythrocytes. Of the 147 hemagglutination-positive allantoic fluids, 82 were AIV positive when confirmed with real-time qRT-PCR. Ten isolates were subtyped as H7N2 (n = 7), H7N1, H1N2, and H2N2. In addition, N subtype could be determined for isolates from an additional 25 samples. These results highlight the fact that screening by real-time qRT-PCR may result in some false-negative cloacal samples for AIV.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country></list><tree><noCountry><name sortKey="Abd El Latif, Hemmat K" sort="Abd El Latif, Hemmat K" uniqKey="Abd El Latif H" first="Hemmat K" last="Abd El Latif">Hemmat K. Abd El Latif</name><name sortKey="Abin, Martha" sort="Abin, Martha" uniqKey="Abin M" first="Martha" last="Abin">Martha Abin</name><name sortKey="Chander, Yogesh" sort="Chander, Yogesh" uniqKey="Chander Y" first="Yogesh" last="Chander">Yogesh Chander</name><name sortKey="El Sayed, Mona A" sort="El Sayed, Mona A" uniqKey="El Sayed M" first="Mona A" last="El Sayed">Mona A. El Sayed</name><name sortKey="Goyal, Sagar M" sort="Goyal, Sagar M" uniqKey="Goyal S" first="Sagar M" last="Goyal">Sagar M. Goyal</name><name sortKey="Raju, Subhatra" sort="Raju, Subhatra" uniqKey="Raju S" first="Subhatra" last="Raju">Subhatra Raju</name><name sortKey="Redig, Patrick T" sort="Redig, Patrick T" uniqKey="Redig P" first="Patrick T" last="Redig">Patrick T. Redig</name></noCountry><country name="États-Unis"><noRegion><name sortKey="El Zowalaty, Mohamed E" sort="El Zowalaty, Mohamed E" uniqKey="El Zowalaty M" first="Mohamed E" last="El Zowalaty">Mohamed E. 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